Analysis Note

Positive ControlHuman colon tissue

Application

ELISA (see coments)Immunoblotting (1-5 g/ml)

General description

Recognizes the ~125 kDa ULK1 protein in human colon tissue.

Purified mouse monoclonal antibody. Recognizes the ~125 kDa ULK1 protein.

This Anti-ULK1 Mouse mAb (2H8) is validated for use in ELISA, Immunoblotting for the detection of ULK1.

Immunogen

A recombinant polypeptide corresponding to amino acids 602-716 of human ULK1, expressed as a GST fusion protein

NA

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)